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0K
BIO

Our Technology

We combine advanced computational methods with cutting-edge experimental techniques to develop novel therapeutic approaches to aging, with a special focus on cell immortalization and transferable longevity factors.

In SilicoStrategyIn VitroImplementationValidation &SequencingRL FeedbackLoopAI-GuidedCRISPR-XEvolution

Data Sources & Fine-Tuning

Advanced AI-driven mutation prediction and target identification using state-of-the-art models and comprehensive databases.

  • Evo2 (ARK Institute) base model for mutation target prediction
  • COSMIC Database integration for oncogenic region avoidance
  • Aging Atlas / GenAge data for pro-longevity loci identification
  • Probability distribution mapping of mutation hotspots

Reinforcement Learning Strategy

Sophisticated RL agents for adaptive mutation strategy generation and optimization based on experimental feedback.

  • Stable Baselines3 PPO/DQN implementation
  • Mutation strategy vector generation
  • Region-specific mutation intensity control
  • Adaptive targeting based on experimental results

Guide RNA Design System

Automated workflow for guide RNA design and synthesis, integrating multiple tools for optimal targeting.

  • n8n workflow automation pipeline
  • CHOPCHOP integration for efficient sgRNA selection
  • Benchling API for commercial-grade synthesis
  • Dual-purpose gRNA pools for mutation and protection
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0K BIO's platform is not just faster evolution—it's smarter evolution. By combining hypermutation, ML-guided constraint design, and synthetic population controls, we solve aging's complexity while preempting risks that nature never had to face.

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