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0K
BIO

Our Technology

We combine advanced computational methods with cutting-edge experimental techniques to develop novel therapeutic approaches to aging, with a special focus on cell immortalization and transferable longevity factors.

In SilicoStrategyIn VitroImplementationValidation &SequencingRL FeedbackLoopAI-GuidedCRISPR-XEvolution

Data Sources & Fine-Tuning

Advanced AI-driven mutation prediction and target identification using state-of-the-art models and comprehensive databases.

  • Evo2 (ARK Institute) base model for mutation target prediction
  • COSMIC Database integration for oncogenic region avoidance
  • Aging Atlas / GenAge data for pro-longevity loci identification
  • Probability distribution mapping of mutation hotspots

Reinforcement Learning Strategy

Sophisticated RL agents for adaptive mutation strategy generation and optimization based on experimental feedback.

  • Stable Baselines3 PPO/DQN implementation
  • Mutation strategy vector generation
  • Region-specific mutation intensity control
  • Adaptive targeting based on experimental results

Guide RNA Design System

Automated workflow for guide RNA design and synthesis, integrating multiple tools for optimal targeting.

  • n8n workflow automation pipeline
  • CHOPCHOP integration for efficient sgRNA selection
  • Benchling API for commercial-grade synthesis
  • Dual-purpose gRNA pools for mutation and protection
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0K BIO's platform is not just faster evolution—it's smarter evolution. By combining hypermutation, ML-guided constraint design, and synthetic population controls, we solve aging's complexity while preempting risks that nature never had to face.

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Research & Innovation

Publications (e.g)

Novel Approaches to Longevity Enhancement Through Synthetic Biology

Kenan Oggad, et al.

Nature Biotechnology2024DOI

A comprehensive study on the application of synthetic biology techniques in longevity research.

Machine Learning Applications in Gene Therapy Optimization

Kenan Oggad, et al.

Cell2023DOI

Novel machine learning approaches for optimizing gene therapy delivery and efficacy.

Patents (e.g)

Method and System for Adaptive Gene Therapy Delivery

Granted

Kenan Oggad, et al.

Filed: 2024-01Patent: US 12,345,678

A novel system for delivering personalized gene therapies using adaptive algorithms.

Artificial Intelligence System for Longevity Pathway Analysis

Pending

Kenan Oggad, et al.

Filed: 2023-06

An AI-driven system for analyzing and identifying key pathways in cellular aging.